|Start position Primer_Fw||20||
Notice that the reverse primer is identical to the negative strand or reverse-complementary to the positive strand.
Notice also that primers are also given in the 5' to 3' orientation, that is, in the orientation that the Taq polymerase is going to start adding nucleotides and extend the PCR product.
|Start position Primer_Rv||402|
|Size of PCR product||383|