T3.1 Here you have the sequence of a gene and two primers to be used in a PCR protocol. Place the primers in the sequence and estimate the expected PCR size.


Start position Primer_Fw 20
Notice that the reverse primer is identical to the negative strand or reverse-complementary to the positive strand.
Notice also that primers are also given in the 5' to 3' orientation, that is, in the orientation that the Taq polymerase is going to start adding nucleotides and extend the PCR product.
Start position Primer_Rv 402
Size of PCR product 383