T3.2 You want to work with this mRNA sequence, to retranscribe it to cDNA.
Design a primer to use in the RT reaction.
Which would be the best of the 4 options below?

This sequence is identical to the mRNA.

So, it will not complement the mRNA, it will not form a double strand hybrid, and it will not be used by the reverse transcriptase to retrotranscribe the mRNA into cDNA.

KEEP TRYING!!

This sequence is complementary to the mRNA. After annealing to the mRNA, the double strand structure will be used by the retrotranscriptase to make a DNA copy of the RNA.

The resulting cDNA will be a full copy of the mRNA molecule.

THIS IS THE BEST OPTION!!

This sequence is complementary to the mRNA. After annealing to the mRNA, the double strand structure will be used by the retrotranscriptase to make a DNA copy of the RNA.

However, the resulting cDNA will not be a full copy of the mRNA molecule (only the first half of the mRNA will be copied).

KEEP TRYING!

This sequence is complementary to the mRNA. After annealing to the mRNA, the double strand structure will be used by the retrotranscriptase to make a DNA copy of the RNA.

However, the resulting cDNA will not be a full copy of the mRNA molecule (only the first 20 nt of the mRNA will be copied).

KEEP TRYING!